Saturday, July 23, 2011

Stereology Workshop


Aug 29, 2011 - Sep 2, 2011 | Bern, Switzerland

Stereology Workshop

An introduction to state-of-the-art stereology with emphasis on its practical application in biomedicine.
Starts on 29/08/2011
Ends on 02/09/2011
Category Stereology
Location Bern, Switzerland

Experimental design, sampling, use of stereological probes, estimation of stereological parameters, and the application of statistical methods appropriate for stereology are covered. The course encompasses lectures, classroom exercises with real biomedical images, and laboratory practicals demonstrating the implementation of proper sampling and processing techniques on real biological organs.

This course is intended for students and researchers from the field of biology and medicine interested in sampling and measurement of 3D structures. Applications involving any kind of microscopy (e.g. light microscopy, confocal laser scanning microscopy, multi-photon microscopy, electron microscopy) as well as non-invasive scanning techniques like CT and NMR are welcome.

Participants should bring their specific projects and questions. During the course, there will be ample opportunity for discussions with the instructors.

Workshop on Quantitative Microscopy 
(in collaboration with the Microscopy Imaging Center (MIC), University of Bern) 

The dates are fixed: 
1. Week: Stereology: August 29 - September 2, 2011
2. Week: Hands-on VISIOPHARM newCAST: September 5 and 6, 2011
(planned)

Stereology provides biomedical researchers with efficient and unbiased tools to obtain real 3D data like volume, surface, length, and number of organs, tissues, cells, and organelles from measurements done on microscopic sections. 
If this is important for your research, this workshop is for you! 

Provisional program (may be subject to change):
1. Week: Stereology
Monday
Presentation of projects by participants 
Lecture: Definition of probes, sampling and basic statistics 
Lecture: Volume estimation, Cavalieri principle 
Exercise: Volume estimation 
Practical: Agar embedding and slicing of organs 

Tuesday
Lecture: The fractionator principle (systematic sampling) 
Exercise: The fractionator 
Lecture: The disector, counting and sampling in 3D 
Exercise: Number estimation 
Lecture: More disector sampling, including connectivity 
Exercise: Connectivity 
Exercise: Alveolar number 

Wednesday
Lecture: Length and Surface, IUR 
Exercise: Length estimation 
Lecture: Ratios, multilevel sampling design, reference trap 
Lecture: Vertical sections 
Exercise: Surface from vertical sections 
Practical: Fractionator sampling, vertical sections; embedding, sectioning and staining 

Thursday
Lecture: Sampling and sizing of particles 
Exercise: The Nucleator 
Exercise: Point sampled intercepts 
Lecture: Tissue deformation, slicing, tools and gadgets 
Demonstration: Stereology and imaging machinery: newCAST 
Demonstration: Stereology web-tool: the STEPanizer 

Friday
Lecture: All the estimators of particle size 
Lunch break 
Final presentation of projects by participants

2. Week: 
Hands-on computer based stereology and imaging machinery: newCAST (Visiopharm, DK)
Two days with practical exercise
PC-Installation for every participant provided. Own notebook may be used.
Study material from participants

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